Nickel Magnetic Beads

      Compared with traditional nickel chromatography media, using Nickel Magnetic Beads to separate and purify histidine-tagged proteins requires no pre-treatment of the crude protein sample (except for inclusion bodies, which need denaturation), and no column chromatography equipment. The process is convenient and offers better cost-effectiveness.

Product Features

1.Low non-specific adsorption

2.Excellent purification efficiency for small sample volumes

3.Suitable for screening experiments with high reproducibility

4.Easy to scale up or down for protein purification

5.High protein binding capacity and high purity of purified protein

Applications

Separation or purification of histidine-tagged proteins

Product Specifications

1.Particle sizes: 200 nm, 1 μm, 3 μm, 5 μm, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 100 μm

2.Concentration: 50 mg/mL

3.Ligand: IDA-Ni

4.Binding capacity: ≥40 mg His-tagged protein / mL (settled bead volume)

5.Storage conditions: 2-8 °C in 20% ethanol

6.Iron (Fe) content: 30%

Protocol / Instructions

1. Binding of beads to protein

Transfer an appropriate amount of beads to a centrifuge tube. Wash three times with Binding Buffer and resuspend in Binding Buffer. Mix the disrupted/lysed bacterial solution with the beads and incubate on a mixer for 30 minutes.

2. Washing the beads

After 30 minutes, place the tube on a magnetic separator to collect the beads. Remove the supernatant for later analysis if desired. Add Washing Buffer to the beads, invert several times to resuspend, then separate magnetically and remove the Washing Buffer for analysis. Add Washing Buffer again to resuspend the beads, then transfer the beads to a new centrifuge tube (to avoid protein contamination). Magnetize, separate, and collect the Washing Buffer.

3. Elution of target protein

Adjust the elution volume as needed to change the concentration of the target protein. Add Elution Buffer, gently invert the tube to resuspend the beads, apply magnetic separation, and collect the eluate in a fresh tube – this is the purified target protein. Repeat the elution several times to ensure complete recovery of the target protein.