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Tosyl Magnetic Beads

Tosyl Magnetic Beads from KBsphere Tech. feature a superparamagnetic Fe₃O₄ core surface-modified with tosyl functional groups. They offer high magnetic content and excellent monodispersity. Under specific reagent conditions, abundant tosyl groups covalently immobilize biological ligands such as proteins onto the bead surface, enabling rapid separation and enrichment of targets from samples. They serve as a critical carrier for research in molecular biology, medicine, and related fields.

Product Features

1.Fast magnetic response and excellent monodispersity

2.Low batch-to-batch variation and high reproducibility

3.Good biocompatibility and low cytotoxicity

4.Easy conjugation with various biomolecules; simple operation

Applications

1.Detection of bioactive substances

2.Sample enrichment and separation

3.Product Parameters & Specifications

4.Particle size: 200 nm, 1 μm, 3 μm, 5 μm, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 100 μm

5.Concentration: 50 mg/mL

6.Storage solution: Isopropanol

7.Surface ligand: Tosyl group

8.Ligand density: ≥ 250 μmol/g

9.Iron (Fe) content: 30%

Biomolecule Conjugation Protocol
1.Take an appropriate amount of beads, wash several times with conjugation buffer to completely remove isopropanol, then separate magnetically for later use.
2.Disperse the desired biomolecule in carbonate buffer (pH 9.0), resuspend the beads in this solution, and conjugate at 37 °C for 2 hours or at 4 °C overnight.
3.After conjugation, separate the beads magnetically and discard the supernatant. Resuspend the beads in carbonate buffer (pH 9.0) containing 1% BSA, then incubate at 25 °C for 1 hour to block unreacted tosyl groups.
4.After blocking, separate the beads magnetically and discard the supernatant. Wash the beads three times with 20 mM PBS buffer (pH 7.4), resuspend, and store at 4 °C. If needed, add 0.02% sodium azide to inhibit bacterial growth.

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