Product Features

· Particle size: 200 nm, 1 μm, 2 μm, 3 μm, 5 μm, 10 μm, 15 μm, 20 μm, 30 μm, 40 μm, 50 μm, 100 μm

· Particle size uniformity: CV < 3%

· Concentration: 50 mg/mL

· Solvent: DI Water

· Surface functional group: Carboxyl group (-COOH)

· Carboxyl density: 200 μmol/g

· Compatible buffers: PBS, Tris-HCl, H₂O

· Iron (Fe) content: 30%

EDC/NHS Bioconjugation Protocol

1. Carboxyl Activation on Magnetic Beads

Transfer mixed magnetic beads to a centrifuge tube and remove supernatant via magnetic separation. Wash the beads 3 times with 20 mM MEST buffer (20 mM MES, pH 5.0, 0.05% Tween 20). Resuspend beads in equal volumes of freshly prepared EDC and NHS solutions, then incubate at 25 °C for 3 hours for activation.

2. Bioconjugation & Blocking

Remove supernatant magnetically. Resuspend beads with biomolecules diluted in pH 8.0 buffer, and incubate at 25 °C for 3 hours or overnight at 4 °C to complete conjugation. Discard supernatant, add buffer containing 1% BSA and resuspend beads. Incubate at 25 °C for 1 hour to block unreacted activated carboxyl groups.

3. Washing & Storage

After blocking, remove supernatant and wash beads 3 times with 20 mM PBS (pH 7.4). Resuspend the beads and store at 4 °C. Add 0.02% sodium azide for antibacterial preservation if needed.

Table 1: Carboxyl Magnetic Beads Series