Product Specifications

· Particle size: 200 nm, 1 μm, 3 μm, 5 μm, 10 μm, 15 μm, 20 μm, 30 μm, 40 μm, 50 μm, 100 μm

· Concentration: 50 mg/mL

· Suspension solution: H₂O (pH ~4.0)

· Amino density: >200 μmol/g

· Compatible buffers: PBS, Tris-HCl, H₂O

· Iron (Fe) content: 30%

Biomolecule Conjugation (Glutaraldehyde Method)

1. Activation

Resuspend beads thoroughly. Take desired volume, wash twice with 20 mM PBS (pH 7.4), remove supernatant by magnetic separation. Add freshly prepared 15% glutaraldehyde, mix well, and react at 25 °C for 1 hour in the dark (keep suspended).

2. Conjugation

Wash activated beads twice with 20 mM PBS (pH 7.4), discard supernatant. Add target biomolecules and disperse in pH 8.0 buffer (0.05% Tween 20 optional for dispersion). React at 25 °C for 3 hours or at 4 °C overnight in the dark (keep uniform suspension).

3. Blocking

Separate conjugated beads magnetically, discard supernatant. Resuspend in 20 mM PBS (pH 7.4) with 5% BSA, block at 25 °C for 1 hour in the dark. Wash and store in 20 mM PBS (pH 7.4) at 4 °C. 0.02% sodium azide can be added for antimicrobial preservation.

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