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Name:Long-Arm NHS-Activated Magnetic Beads

Tel:15251417609

Details

      KBsphere® Long-Arm NHS-Activated Magnetic Beads are densely functionalized with Nhydroxysuccinimide (NHS) groups on the bead surface. They rapidly and efficiently covalently conjugate to any primary amine-containing ligand via formation of stable amide bonds. The coupling reaction proceeds under mild conditions: 15–30 minutes at room temperature (pH 6.5–9), or 4 hours at 4 °C. Featuring a hydrophilic 21-atom spacer arm, these beads effectively reduce steric hindrance, making them particularly suitable for proteins.

These pre-activated, ready-to-use magnetic beads offer straightforward handling. Rapid coupling is achievable at room temperature within the pH range of 6.5–9. The resulting covalent bonds are stable, enabling repeated use of the beads with an extremely low non-specific binding rate. As an affinity purification medium, NHS-activated magnetic beads excel at capturing target molecules, cells or subcellular components from complex samples. They are widely applied in the purification of antibodies, proteins/peptides, DNA/RNA, as well as cell sorting and immunoprecipitation assays, serving as an efficient and reliable immunoaffinity matrix.

Product Specifications

1.Particle size: 200 nm, 1 μm, 3 μm, 5 μm, 10 μm, 20 μm, 30 μm, 40 μm, 50 μm, 100 μm

2.Concentration: 50 mg/mL

3.Storage solution: Isopropanol

4.Ligand: NHS

5.Ligand density: 200–300 μmol/g

6.Storage condition: 2–8 °C

7.Iron (Fe) content: 30%

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